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How to use PETG media bottles to dispense serum

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When culturing cells, serum is an essential nutrient that increases adhesion factors, growth factors, binding proteins, etc. for cell growth. When using serum, we will involve the dispensing operation of serum, so how should it be dispensed into PETG media bottles ? 1. Thawing Take the serum out of the refrigerator at -20 degrees, thaw at room temperature (or immerse in tap water) (it takes about 30 minutes to 2 hours, or put it in a refrigerator at 4 degrees overnight; if it is not inactivated immediately after thawing, you can put it in 4 Store temporarily in the refrigerator). 2. Inactivation Water bath, 56°C, 30 minutes, and shake evenly at any time. Take it out, put it on ice immediately, and let it cool down to room temperature naturally (about 1-3 hours). During the heat inactivation process, proper shaking at regular intervals can reduce the occurrence of precipitation. 125ml Square PETG Media Bottles 3. Packing Transfer to the aseptic room, put the serum into 50-100ml PETG me...
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What is the precipitate in the PETG media bottle?

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PETG media bottle is a special packaging container for storing serum. Sometimes we find some sediment inside the bottle, so what exactly are these sediments? These substances are mainly calcium phosphate or fibrin. 1. Calcium phosphate A common sediment that appears as a cloud. Observing through an inverted microscope, you will find many small black spots in the PETG media bottle. These black spots are free to move due to Brownian motion and are therefore often mistaken for microbial contamination. A small amount of precipitation is a normal phenomenon during the thawing process of serum. Too clear serum does not represent higher quality, and it may also be the result of repeated freezing and thawing during processing to remove fibrinogen as much as possible. 125ml-Square-PETG-PET-Media-Bottles 2. Fibrin Larger material (up to 1-2 mm) visible to the naked eye. Serum is collected at low temperatures and processed rapidly, resulting in some fibrinogen remaining in solution. Although mo...
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